Efficacy of DNA sequencing on samples of terrestrial invertebrates 2018-19

Invertebrate samples collected using Natural England survey protocols will be analysed using appropriate DNA techniques, to provide a comparison to traditional techniques (data will be supplied). Information will be collected to ascertain the following for each of the two methods:

• How reliable and accurate is the DNA species identification?
• How much does each method cost (in time and money)?
• How long does the identification process take?
• Can abundance be inferred as part of DNA sequencing?

In addition, the following questions should be addressed:

• How reliable is BOLD, or other databases available to the contractor, for undertaking invertebrate identification? A reference collection will not be made available.
• Is there a need to review collection and storage methods for DNA sequencing of terrestrial invertebrates?

Additional information:

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